Cystatin A protease inhibitor and cysteine proteases in human cervicovaginal fluid in term pregnancy and labor

YJ Heng; MKW Di Quinzio; M Permezel; GE Rice; HM Georgiou

Journal article

Cystatin A protease inhibitor and cysteine proteases in human cervicovaginal fluid in term pregnancy and labor

YJ Heng, MKW Di Quinzio, M Permezel, GE Rice, HM Georgiou

American Journal of Obstetrics and Gynecology | MOSBY-ELSEVIER | Published : 2011

DOI: 10.1016/j.ajog.2010.10.912

Abstract

Objective The purpose of this study was to investigate the temporal changes in immunoreactive cystatin A and the enzymatic activity of cathepsins B, H, L, and S in human cervicovaginal fluid (CVF) in late pregnancy and spontaneous labor. Study Design CVF was collected weekly (n = 95 women) from 36 weeks gestation until spontaneous term labor. Cystatin A was quantified using enzyme-linked immunosorbent assay. The enzyme activity of cathepsins B, H, L, and S was measured with fluorometric enzyme assay kits. Results Cystatin A significantly decreased towards (P = .016, 2-way analysis of variance) and during labor (P < .001, 2-way analysis of variance). Enzymatic activity of cathepsins B, H, and..

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University of Melbourne Researchers

Megan Di Quinzio Author

Michael Permezel Author

Harry Georgiou Author

Related Projects (2)

2007 DEVELOPMENT GRANT - (DEVELOPMENT OF A MULTIPLEX ASSAY FOR THE IDENTIFICATION OF WOMEN AT RISK OF PRETERM LABOUR)

Preterm birth (ie birth before the 37th week of gestation) is the most significant problem facing contemporary clinical obstetrics in the de..

IDENTIFICATION OF NOVEL BIOMARKERS PREDICTIVE OF IMPENDING PRETERM LABOUR

Grants

Awarded by National Health and Medical Research Council

Funding Acknowledgements

Supported by a National Health and Medical Research Council of Australia (NHMRC) Development Grant (454451) and the Medical Research Foundation for Women and Babies. YJ Heng is a recipient of an NHMRC Biomedical (Dora Lush) Postgraduate Research Scholarship (454880).